Cascading capsules

A one-pot chemical reaction system based on compartmentalizing three different enzymes in a porous polymer membrane has been designed and tested by Jan van Hest and Alan Rowan of the Nijmegen University in The Netherlands. The system allows the team to cascade the reaction of an acetylated glucose molecule via reaction by lipase B to free glucose which is then oxidized on contact with glucose oxidase and the product of this reaction is then used to release hydrogen peroxide using horseradish peroxidase. This proof of principle demonstrate the potential of undertaking biotransformation in a one-pot cascade reaction without time-consuming and wasteful separation and purification stages.